Analysis and Quantitation of Glycated Hemoglobin by Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry.

Autor: Hattan SJ; SimulTOF Systems, Sudbury, MA, 01776, USA. stephen.hattan@simultof.com., Parker KC; SimulTOF Systems, Sudbury, MA, 01776, USA., Vestal ML; SimulTOF Systems, Sudbury, MA, 01776, USA., Yang JY; Department of Pathology, University of California San Diego, La Jolla, CA, 92093-0612, USA., Herold DA; Department of Pathology, University of California San Diego, La Jolla, CA, 92093-0612, USA.; VA San Diego Healthcare System, PALMS, MS 113, San Diego, CA, 92161, USA., Duncan MW; Division of Endocrinology, Metabolism, and Diabetes, University of Colorado School of Medicine, MS 8106, Aurora, CO, 80045, USA.
Jazyk: angličtina
Zdroj: Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2016 Mar; Vol. 27 (3), pp. 532-41. Date of Electronic Publication: 2016 Jan 05.
DOI: 10.1007/s13361-015-1316-6
Abstrakt: Measurement of glycated hemoglobin is widely used for the diagnosis and monitoring of diabetes mellitus. Matrix assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometry (MS) analysis of patient samples is used to demonstrate a method for quantitation of total glycation on the β-subunit of hemoglobin. The approach is accurate and calibrated with commercially available reference materials. Measurements were linear (R(2) > 0.99) across the clinically relevant range of 4% to 20% glycation with coefficients of variation of ≤ 2.5%. Additional and independent measurements of glycation of the α-subunit of hemoglobin are used to validate β-subunit glycation measurements and distinguish hemoglobin variants. Results obtained by MALDI-TOF MS were compared with those obtained in a clinical laboratory using validated HPLC methodology. MALDI-TOF MS sample preparation was minimal and analysis times were rapid making the method an attractive alternative to methodologies currently in practice.
Databáze: MEDLINE
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