Development and Biological Evaluation of Inkjet Printed Drug Coatings on Intravascular Stent.

Autor:	Scoutaris N; School of Sciences, Faculty of Engineering and Science, University of Greenwich , Medway, Kent ME4 4TB, U.K., Chai F; Université Lille Nord de France , 590000 Lille, France.; INSERM U1008, Groupe Recherche Biomatériaux, Faculty of Medicine, Université Lille 2 , 59045 Lille, France., Maurel B; Université Lille Nord de France , 590000 Lille, France.; INSERM U1008, Groupe Recherche Biomatériaux, Faculty of Medicine, Université Lille 2 , 59045 Lille, France., Sobocinski J; Université Lille Nord de France , 590000 Lille, France.; INSERM U1008, Groupe Recherche Biomatériaux, Faculty of Medicine, Université Lille 2 , 59045 Lille, France., Zhao M; UCL School of Pharmacy, University College London , 29-39 Brunswick Square, London WC1 N 1AX, U.K., Moffat JG; UCL School of Pharmacy, University College London , 29-39 Brunswick Square, London WC1 N 1AX, U.K., Craig DQ; UCL School of Pharmacy, University College London , 29-39 Brunswick Square, London WC1 N 1AX, U.K., Martel B; Université Lille Nord de France , 590000 Lille, France.; UMET CNRS 8207, Équipe Ingénierie des Systèmes Polymères, University Lille 1 , 59655 Villeneuve d'Ascq, France., Blanchemain N; Université Lille Nord de France , 590000 Lille, France.; INSERM U1008, Groupe Recherche Biomatériaux, Faculty of Medicine, Université Lille 2 , 59045 Lille, France., Douroumis D; School of Sciences, Faculty of Engineering and Science, University of Greenwich , Medway, Kent ME4 4TB, U.K.
Jazyk:	angličtina
Zdroj:	Molecular pharmaceutics [Mol Pharm] 2016 Jan 04; Vol. 13 (1), pp. 125-33. Date of Electronic Publication: 2015 Dec 03.
DOI:	10.1021/acs.molpharmaceut.5b00570
Abstrakt:	Inkjet-printing technology was used to apply biodegradable and biocompatible polymeric coatings of poly(d,l-lactide) with the antiproliferative drugs simvastatin (SMV) and paclitaxel (PCX) on coronary metal stents. A piezoelectric dispenser applied coating patterns of very fine droplets (300 pL) and inkjet printing was optimized to develop uniform, accurate and reproducible coatings of high yields on the stent strut. The drug loaded polymeric coatings were assed by scanning electron microscopy (SEM), atomic force microscopy (AFM), and transition thermal microscopy (TTM) where a phase separation was observed for SMV/PLA layers while PCX showed a uniform distribution within the polymer layers. Cytocompatibility studies of PLA coatings showed excellent cell adhesion with no decrease of cell viability and proliferation. In vivo stent implantation studies showed significant intrastent restenosis (ISR) for PCX/PLA and PLA plain coatings similar to marketed Presillion (bare metal) and Cypher (drug eluting) stents. The investigation of several cytokine levels after 7 days of stent deployment showed no inflammatory response and hence no in vivo cytotoxicity related to PLA coatings. Inkjet printing can be employed as a robust coating technology for the development of drug eluting stents compared to the current conventional approaches.
Databáze:	MEDLINE
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