Quantitative analysis of unconjugated and total bisphenol A in human urine using solid-phase extraction and UPLC-MS/MS: method implementation, method qualification and troubleshooting.

Autor: Buscher B; TNO Triskelion BV, Utrechtseweg 48, Zeist, The Netherlands., van de Lagemaat D; TNO Triskelion BV, Utrechtseweg 48, Zeist, The Netherlands., Gries W; Currenta GmbH & Co., OHG, Leverkusen, Germany., Beyer D; Bayer Pharma Aktiengesellschaft, Wuppertal, Germany. Electronic address: dieter.beyer@bayer.com., Markham DA; The Dow Chemical Company, Midland, MI, USA., Budinsky RA; The Dow Chemical Company, Midland, MI, USA., Dimond SS; Saudi Basic Industries Corporation (SABIC), Pittsfield, MA, USA., Nath RV; SABIC Research & Technology Pvt., Ltd., Bangalore, India., Snyder SA; Covestro LLC, Pittsburgh, PA, USA., Hentges SG; American Chemistry Council, Washington DC, USA.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2015 Nov 15; Vol. 1005, pp. 30-8. Date of Electronic Publication: 2015 Sep 26.
DOI: 10.1016/j.jchromb.2015.09.020
Abstrakt: The aim of the presented investigation was to document challenges encountered during implementation and qualification of a method for bisphenol A (BPA) analysis and to develop and discuss precautions taken to avoid and to monitor contamination with BPA during sample handling and analysis. Previously developed and published HPLC-MS/MS methods for the determination of unconjugated BPA (Markham et al. Journal of Analytical Toxicology, 34 (2010) 293-303) [17] and total BPA (Markham et al. Journal of Analytical Toxicology, 38 (2014) 194-203) [20] in human urine were combined and transferred into another laboratory. The initial method for unconjugated BPA was developed and evaluated in two independent laboratories simultaneously. The second method for total BPA was developed and evaluated in one of these laboratories to conserve resources. Accurate analysis of BPA at sub-ppb levels is a challenging task as BPA is a widely used material and is ubiquitous in the environment at trace concentrations. Propensity for contamination of biological samples with BPA is reported in the literature during sample collection, storage, and/or analysis. Contamination by trace levels of BPA is so pervasive that even with extraordinary care, it is difficult to completely exclude the introduction of BPA into biological samples and, consequently, contamination might have an impact on BPA biomonitoring data. The applied UPLC-MS/MS method was calibrated from 0.05 to 25ng/ml. The limit of quantification was 0.1ng/ml for unconjugated BPA and 0.2ng/ml for total BPA, respectively, in human urine. Finally, the method was applied to urine samples derived from 20 volunteers. Overall, BPA can be analyzed in human urine with acceptable recovery and repeatability if sufficient measures are taken to avoid contamination throughout the procedure from sample collection until UPLC-MS/MS analysis.
(Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: