Antitumour Activity of the Microencapsulation of Annona vepretorum Essential Oil.

Autor: Bomfim LM; Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil., Menezes LR; Department of Chemistry, Federal University of Sergipe, São Cristóvão, Brazil., Rodrigues AC; Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil., Dias RB; Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil., Rocha CA; Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.; Department of Propedeutics, Federal University of Bahia, Salvador, Brazil., Soares MB; Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.; Center of Biotechnology and Cell Therapy, Hospital São Rafael, Salvador, Brazil., Neto AF; Faculty of Pharmacy, Federal University of Bahia, Salvador, Brazil., Nascimento MP; Northeastern Center for Strategic Technologies (CETENE), Recife, Brazil., Campos AF; Northeastern Center for Strategic Technologies (CETENE), Recife, Brazil., Silva LC; Faculty of Pharmacy, Federal University of Bahia, Salvador, Brazil., Costa EV; Department of Chemistry, Federal University of Amazonas, Manaus, Brazil., Bezerra DP; Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.
Jazyk: angličtina
Zdroj: Basic & clinical pharmacology & toxicology [Basic Clin Pharmacol Toxicol] 2016 Mar; Vol. 118 (3), pp. 208-13. Date of Electronic Publication: 2015 Oct 06.
DOI: 10.1111/bcpt.12488
Abstrakt: Annona vepretorum Mart. (Annonaceae), popularly known as 'bruteira', has nutritional and medicinal uses. This study investigated the chemical composition and antitumour potential of the essential oil of A. vepretorum leaf alone and complexed with β-cyclodextrin in a microencapsulation. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus and analysed using GC-MS and GC-FID. In vitro cytotoxicity of the essential oil and some of its major constituents in tumour cell lines from different histotypes was evaluated using the alamar blue assay. Furthermore, the in vivo efficacy of essential oil was demonstrated in mice inoculated with B16-F10 mouse melanoma. The essential oil included bicyclogermacrene (35.71%), spathulenol (18.89%), (E)-β-ocimene (12.46%), α-phellandrene (8.08%), o-cymene (6.24%), germacrene D (3.27%) and α-pinene (2.18%) as major constituents. The essential oil and spathulenol exhibited promising cytotoxicity. In vivo tumour growth was inhibited by the treatment with the essential oil (inhibition of 34.46%). Importantly, microencapsulation of the essential oil increased in vivo tumour growth inhibition (inhibition of 62.66%).
(© 2015 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).)
Databáze: MEDLINE
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