The Legionella Anti-autophagy Effector RavZ Targets the Autophagosome via PI3P- and Curvature-Sensing Motifs.

Autor:	Horenkamp FA; Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA., Kauffman KJ; Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA., Kohler LJ; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520, USA., Sherwood RK; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520, USA., Krueger KP; Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA., Shteyn V; Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA., Roy CR; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520, USA., Melia TJ; Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA. Electronic address: thomas.melia@yale.edu., Reinisch KM; Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA. Electronic address: karin.reinisch@yale.edu.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2015 Sep 14; Vol. 34 (5), pp. 569-76. Date of Electronic Publication: 2015 Sep 03.
DOI:	10.1016/j.devcel.2015.08.010
Abstrakt:	Autophagy is a conserved membrane transport pathway used to destroy pathogenic microbes that access the cytosol of cells. The intracellular pathogen Legionella pneumophila interferes with autophagy by delivering an effector protein, RavZ, into the host cytosol. RavZ acts by cleaving membrane-conjugated Atg8/LC3 proteins from pre-autophagosomal structures. Its remarkable efficiency allows minute quantities of RavZ to block autophagy throughout the cell. To understand how RavZ targets pre-autophagosomes and specifically acts only on membrane-associated Atg8 proteins, we elucidated its structure. Revealed is a catalytic domain related in fold to Ulp family deubiquitinase-like enzymes and a C-terminal PI3P-binding module. RavZ targets the autophagosome via the PI3P-binding module and a catalytic domain helix, and it preferentially binds high-curvature membranes, intimating localization to highly curved domains in autophagosome intermediate membranes. RavZ-membrane interactions enhance substrate affinity, providing a mechanism for interfacial activation that also may be used by host autophagy proteins engaging only lipidated Atg8 proteins. (Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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