Tungsten-induced carcinogenesis in human bronchial epithelial cells.

Autor: Laulicht F; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA., Brocato J; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA., Cartularo L; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA., Vaughan J; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA., Wu F; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA., Kluz T; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA., Sun H; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA., Oksuz BA; Genome Technology Center, New York University Langone Medical Center, New York, NY 10016, USA., Shen S; Center for Health Informatics and Bioinformatics, New York University Langone Medical Center, New York, NY 10016, USA., Peana M; Department of Chemistry and Pharmacy, University of Sassari, Sassari, Italy., Medici S; Department of Chemistry and Pharmacy, University of Sassari, Sassari, Italy., Zoroddu MA; Department of Chemistry and Pharmacy, University of Sassari, Sassari, Italy., Costa M; Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY 10987, USA. Electronic address: Max.Costa@nyumc.org.
Jazyk: angličtina
Zdroj: Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2015 Oct 01; Vol. 288 (1), pp. 33-9. Date of Electronic Publication: 2015 Jul 09.
DOI: 10.1016/j.taap.2015.07.003
Abstrakt: Metals such as arsenic, cadmium, beryllium, and nickel are known human carcinogens; however, other transition metals, such as tungsten (W), remain relatively uninvestigated with regard to their potential carcinogenic activity. Tungsten production for industrial and military applications has almost doubled over the past decade and continues to increase. Here, for the first time, we demonstrate tungsten's ability to induce carcinogenic related endpoints including cell transformation, increased migration, xenograft growth in nude mice, and the activation of multiple cancer-related pathways in transformed clones as determined by RNA sequencing. Human bronchial epithelial cell line (Beas-2B) exposed to tungsten developed carcinogenic properties. In a soft agar assay, tungsten-treated cells formed more colonies than controls and the tungsten-transformed clones formed tumors in nude mice. RNA-sequencing data revealed that the tungsten-transformed clones altered the expression of many cancer-associated genes when compared to control clones. Genes involved in lung cancer, leukemia, and general cancer genes were deregulated by tungsten. Taken together, our data show the carcinogenic potential of tungsten. Further tests are needed, including in vivo and human studies, in order to validate tungsten as a carcinogen to humans.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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