Potential DMSP-degrading Roseobacter clade dominates endosymbiotic microflora of Pyrodinium bahamense var. compressum (Dinophyceae) in vitro.

Autor: Onda DF; The Marine Science Institute, University of the Philippines, 1101, Diliman, Quezon City, Philippines., Azanza RV, Lluisma AO
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2015 Sep; Vol. 197 (7), pp. 965-71. Date of Electronic Publication: 2015 Jul 05.
DOI: 10.1007/s00203-015-1133-0
Abstrakt: Many aspects of the biology and ecology of the toxic dinoflagellate Pyrodinium bahamense var. compressum are still poorly understood. In this brief note, we present identification of its associated intracellular bacteria or endosymbionts via PCR cloning and 16s rRNA gene sequencing and their localization by confocal microscopy, a first for Pyrodinium. The most frequently observed species in the endosymbiotic microflora were from Roseobacter clade (Alphaproteobacteria, 68%) and Gilvibacter sediminis (Flavobacteriaceae, 20%). Roseobacter lineage, the most abundant taxa in this study, is known to be involved in dimethylsulfoniopropionate metabolism which is highly produced in dinoflagellates-a possible strong factor shaping the structure of the associated bacterial community.
Databáze: MEDLINE