Protective Immunity and Reduced Renal Colonization Induced by Vaccines Containing Recombinant Leptospira interrogans Outer Membrane Proteins and Flagellin Adjuvant.

Autor:	Monaris D; Laboratório de Bacteriologia, Instituto Butantan, São Paulo, SP, Brazil Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil., Sbrogio-Almeida ME; Centro de Biotecnologia, Instituto Butantan, São Paulo, SP, Brazil., Dib CC; Laboratório de Tuberculose, Instituto Biológico, São Paulo, SP Brazil., Canhamero TA; Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, Brazil., Souza GO; Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil., Vasconcellos SA; Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil., Ferreira LC; Laboratório de Desenvolvimento de Vacinas, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil., Abreu PA; Laboratório de Bacteriologia, Instituto Butantan, São Paulo, SP, Brazil patricia.aniz@butantan.gov.br.
Jazyk:	angličtina
Zdroj:	Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2015 Aug; Vol. 22 (8), pp. 965-73. Date of Electronic Publication: 2015 Jun 24.
DOI:	10.1128/CVI.00285-15
Abstrakt:	Leptospirosis is a global zoonotic disease caused by different Leptospira species, such as Leptospira interrogans, that colonize the renal tubules of wild and domestic animals. Thus far, attempts to develop effective leptospirosis vaccines, both for humans and animals, have failed to induce immune responses capable of conferring protection and simultaneously preventing renal colonization. In this study, we evaluated the protective immunity induced by subunit vaccines containing seven different recombinant Leptospira interrogans outer membrane proteins, including the carboxy-terminal portion of the immunoglobulinlike protein A (LigA(C)) and six novel antigens, combined with aluminum hydroxide (alum) or Salmonella flagellin (FliC) as adjuvants. Hamsters vaccinated with the different formulations elicited high antigen-specific antibody titers. Immunization with LigA(C), either with alum or flagellin, conferred protective immunity but did not prevent renal colonization. Similarly, animals immunized with LigA(C) or LigA(C) coadministered with six leptospiral proteins with alum adjuvant conferred protection but did not reduce renal colonization. In contrast, immunizing animals with the pool of seven antigens in combination with flagellin conferred protection and significantly reduced renal colonization by the pathogen. The present study emphasizes the relevance of antigen composition and added adjuvant in the efficacy of antileptospirosis subunit vaccines and shows the complex relationship between immune responses and renal colonization by the pathogen. (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
Databáze:	MEDLINE
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