Keratoconjunctivitis in a group of Icelandic horses with suspected γ-herpesvirus involvement.

Autor: Rushton JO; Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Austria., Kolodziejek J; Institute of Virology, University of Veterinary Medicine Vienna, Austria., Nell B; Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Austria., Weissenböck H; Institute of Pathology and Forensic Veterinary Medicine, University of Veterinary Medicine Vienna, Austria., Nowotny N; Institute of Virology, University of Veterinary Medicine Vienna, Austria.; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman.
Jazyk: angličtina
Zdroj: Equine veterinary journal [Equine Vet J] 2016 Jul; Vol. 48 (4), pp. 427-9. Date of Electronic Publication: 2015 Aug 21.
DOI: 10.1111/evj.12465
Abstrakt: Reasons for Performing Study: The role of equid γ-herpesviruses on ocular surface diseases has been disputed, because the diagnosis is usually based on clinical symptoms and detection of viral DNA from samples obtained from live animals.
Objectives: To describe the clinical course, results of polymerase chain reaction (PCR) analysis, in situ hybridisation, cell culture and pathohistological findings of select cases in a presumed outbreak of herpesvirus infection in a group of 15 Icelandic horses.
Study Design: Case series.
Methods: Pooled ocular and nasal swabs and peripheral blood mononuclear cells of horses diagnosed clinically with herpesvirus-associated keratoconjunctivitis were analysed for presence of equine herpesviruses (EHV)-2 and EHV-5 nucleic acid using real-time PCR. Necropsy specimens from one horse, subjected to euthanasia due to deterioration of clinical symptoms were examined histopathologically, and analysed for presence of EHV-2 and EHV-5 nucleic acid using real-time PCR. In situ hybridisation and cell culture of select samples were performed.
Results: All horses with symptoms of severe keratoconjunctivitis were positive for presence of either EHV-2 and/or EHV-5 nucleic acid using real-time PCR. Assessment of necropsy specimens of the most severely affected case, revealed presence of EHV-2 and/or EHV-5 nucleic acid in several ocular and extraocular anatomical locations. The remaining horses responded favourably to symptomatic treatment.
Conclusions: This case series illustrates a severe outbreak of keratoconjunctivitis in a group of Icelandic horses, with suspected γ-herpesvirus involvement. For the first time equid γ-herpesviruses were detected in intraocular anatomical locations.
(© 2015 EVJ Ltd.)
Databáze: MEDLINE
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