The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup.

Autor: Gollapudi BB; E(x)ponent , Midland, MI, USA., Lynch AM; GlaxoSmithKline, Hertfordshire, UK., Heflich RH; US Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR, USA. Electronic address: robert.heflich@fda.hhs.gov., Dertinger SD; Litron Laboratories, Rochester, NY, USA., Dobrovolsky VN; US Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR, USA., Froetschl R; Federal Institute for Drugs and Medical Devices, Bonn, Germany., Horibata K; National Institute of Health Sciences, Tokyo, Japan., Kenyon MO; Pfizer Global Research and Development, Groton, CT, USA., Kimoto T; Teijin Pharma, Tokyo, Japan., Lovell DP; St. George's, University of London, UK., Stankowski LF Jr; BioReliance, Rockville, MD, USA., White PA; Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada., Witt KL; National Institutes of Health, National Institute of Environmental Health Sciences, Division of the National Toxicology Program, Research Triangle Park, NC, USA., Tanir JY; Health and Environmental Sciences Institute, Washington, DC, USA.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2015 May 01; Vol. 783, pp. 23-35. Date of Electronic Publication: 2014 Sep 26.
DOI: 10.1016/j.mrgentox.2014.09.007
Abstrakt: The in vivo Pig-a assay uses flow cytometry to measure phenotypic variants for antibody binding to cell surface glycosylphosphatidylinositol (GPI)-anchored proteins. There is good evidence suggesting that the absence of antibody binding is the result of a mutation in the endogenous X-linked Pig-a gene, which forms the rationale for the assay. Although the assay has been performed with several types of hematopoietic cells and in a variety of mammalian species, including humans, currently it is optimized only for measuring CD59-deficient (presumed Pig-a mutant) erythrocytes in the peripheral blood of rats. An expert workgroup formed by the International Workshop on Genotoxicity Testing considered the state of assay development and the potential of the assay for regulatory use. Consensus was reached on what is known about the Pig-a assay and how it should be conducted, and recommendations were made on additional data and refinements that would help to further enhance the assay for use in hazard identification and risk assessment.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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