Polyethylene glycol protects primary hepatocytes during supercooling preservation.

Autor: Puts CF; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA., Berendsen TA; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA., Bruinsma BG; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA., Ozer S; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA., Luitje M; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA., Usta OB; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA., Yarmush ML; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA; Department of Biomedical Engineering, Rutgers University, Piscataway, NJ, USA. Electronic address: ireis@sbi.org., Uygun K; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, USA. Electronic address: uygun.korkut@mgh.harvard.edu.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2015 Aug; Vol. 71 (1), pp. 125-9. Date of Electronic Publication: 2015 May 01.
DOI: 10.1016/j.cryobiol.2015.04.010
Abstrakt: Cold storage (at 4°C) offers a compromise between the benefits and disadvantages of cooling. It allows storage of organs or cells for later use that would otherwise quickly succumb to warm ischemia, but comprises cold ischemia that, when not controlled properly, can result in severe damage as well by both similar and unique mechanisms. We hypothesized that polyethylene glycol (PEG) 35 kDa would ameliorate these injury pathways and improve cold primary hepatocyte preservation. We show that reduction of the storage temperature to below zero by means of supercooling, or subzero non-freezing, together with PEG supplementation increases the viable storage time of primary rat hepatocytes in University of Wisconsin (UW) solution from 1 day to 4 days. We find that the addition of 5% PEG 35 kDa to the storage medium prevents cold-induced lipid peroxidation and maintains hepatocyte viability and functionality during storage. These results suggest that PEG supplementation in combination with supercooling may enable a more optimized cell and organ preservation.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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