| Autor: |
El-Gendy R; 1 Biomaterials and Tissue Engineering Group, Department of Oral Biology, University of Leeds, St. James's University Hospital , Leeds, United Kingdom .; 2 Biomineralisation Group, Department of Oral Biology, University of Leeds, St. James's University Hospital , Leeds, United Kingdom .; 3 Department of Oral Pathology, Faculty of Dentistry, Suez Canal University , Ismailia, Egypt ., Kirkham J; 2 Biomineralisation Group, Department of Oral Biology, University of Leeds, St. James's University Hospital , Leeds, United Kingdom ., Newby PJ; 4 Department of Materials, Imperial College London , London, United Kingdom ., Mohanram Y; 1 Biomaterials and Tissue Engineering Group, Department of Oral Biology, University of Leeds, St. James's University Hospital , Leeds, United Kingdom ., Boccaccini AR; 4 Department of Materials, Imperial College London , London, United Kingdom .; 5 Institute of Biomaterials, University of Erlangen-Nuremberg , Erlangen, Germany ., Yang XB; 1 Biomaterials and Tissue Engineering Group, Department of Oral Biology, University of Leeds, St. James's University Hospital , Leeds, United Kingdom . |
| Abstrakt: |
Identification of a suitable cell source combined with an appropriate 3D scaffold is an essential prerequisite for successful engineering of skeletal tissues. Both osteogenesis and angiogenesis are key processes for bone regeneration. This study investigated the vascularization potential of a novel combination of human dental pulp stromal cells (HDPSCs) with 45S5 Bioglass® scaffolds for tissue-engineered mineral constructs in vivo and in vitro. 45S5 Bioglass scaffolds were produced by the foam replication technique with the standard composition of 45 wt% SiO2, 24.5 wt% Na2O, 24.5 wt% CaO, and 6 wt% P2O5. HDPSCs were cultured in monolayers and on porous 45S5 Bioglass scaffolds under angiogenic and osteogenic conditions for 2-4 weeks. HDPSCs expressed endothelial gene markers (CD34, CD31/PECAM1, and VEGFR2) under both conditions in the monolayer. A combination of HDPSCs with 45S5 Bioglass enhanced the expression of these gene markers. Positive immunostaining for CD31/PECAM1 and VEGFR2 and negative staining for CD34 supported the gene expression data, while histology revealed evidence of endothelial cell-like morphology within the constructs. More organized tubular structures, resembling microvessels, were seen in the constructs after 8 weeks of implantation in vivo. In conclusion, this study suggests that the combination of HDPSCs with 45S5 Bioglass scaffolds offers a promising strategy for regenerating vascularized bone grafts. |
