Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots.

Autor: Nally MC; IBT, Instituto de Biotecnología-Facultad de Ingeniería-Universidad Nacional de San Juan, Av. Libertador San Martín 1109 oeste, CP5400 San Juan,Argentina. Electronic address: cristinanally@yahoo.com.ar., Pesce VM; IBT, Instituto de Biotecnología-Facultad de Ingeniería-Universidad Nacional de San Juan, Av. Libertador San Martín 1109 oeste, CP5400 San Juan,Argentina., Maturano YP; IBT, Instituto de Biotecnología-Facultad de Ingeniería-Universidad Nacional de San Juan, Av. Libertador San Martín 1109 oeste, CP5400 San Juan,Argentina., Rodriguez Assaf LA; IBT, Instituto de Biotecnología-Facultad de Ingeniería-Universidad Nacional de San Juan, Av. Libertador San Martín 1109 oeste, CP5400 San Juan,Argentina., Toro ME; IBT, Instituto de Biotecnología-Facultad de Ingeniería-Universidad Nacional de San Juan, Av. Libertador San Martín 1109 oeste, CP5400 San Juan,Argentina., Castellanos de Figueroa LI; PROIMI, Planta Piloto de Procesos Industriales Microbiológicos, Av. Belgrano y Pasaje Caseros, CP4000 Tucumán, Argentina; FBQyF-UNT, Facultad de Bioquímica, Química y Farmacia, Ayacucho 455,CP4000 Tucumán, Argentina., Vazquez F; IBT, Instituto de Biotecnología-Facultad de Ingeniería-Universidad Nacional de San Juan, Av. Libertador San Martín 1109 oeste, CP5400 San Juan,Argentina.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2015 Jul 02; Vol. 204, pp. 91-100. Date of Electronic Publication: 2015 Mar 27.
DOI: 10.1016/j.ijfoodmicro.2015.03.024
Abstrakt: The aim of this study was to determine the putative modes of action of 59 viticultural yeasts (31 Saccharomyces and 28 non-Saccharomyces) that inhibited fungi isolated from sour and grey rot in grapes. Inhibition of fungal mycelial growth by metabolites, enzyme activities (laminarinases, chitinases), antifungal volatiles, competition for nutrients (siderophores, Niche Overlap Index (NOI)), inhibition of fungal spore germination and decreased germinal tube length and induction of resistance were assayed. Biofungicide yeasts were classified into "antifungal patterns", according to their mechanisms of action. Thirty isolates presented at least two of the mechanisms assayed. We propose that inhibition of fungal mycelial growth by metabolites, laminarinases, competition for nutrients, inhibition of fungal spore germination and decreased germinal tube length, and antifungal volatiles by Saccharomyces and non-Saccharomyces viticultural yeasts is used as putative biocontrol mechanisms against phytopathogenic fungi. Twenty-four different antifungal patterns were identified. Siderophore production (N)and a combination of siderophore production and NOI>0.92 (M)were the most frequent antifungal patterns observed in the biofungicide yeasts assayed. Elucidation of these mechanisms could be useful for optimization of an inoculum formulation, resulting in a more consistent control of grey and sour rot with Saccharomyces and non-Saccharomyces biocontrol yeasts.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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