Analysis of phenazepam and 3-hydroxyphenazepam in post-mortem fluids and tissues.

Autor: Crichton ML; Centre for Forensic and Legal Medicine, University of Dundee, Dundee, DD1 4HN, Scotland, UK., Shenton CF; Centre for Forensic and Legal Medicine, University of Dundee, Dundee, DD1 4HN, Scotland, UK., Drummond G; Centre for Forensic and Legal Medicine, University of Dundee, Dundee, DD1 4HN, Scotland, UK., Beer LJ; Centre for Forensic and Legal Medicine, University of Dundee, Dundee, DD1 4HN, Scotland, UK., Seetohul LN; Centre for Forensic and Legal Medicine, University of Dundee, Dundee, DD1 4HN, Scotland, UK.; Department of Chemical and Forensic Sciences, University of Huddersfield, Huddersfield, UK., Maskell PD; Centre for Forensic and Legal Medicine, University of Dundee, Dundee, DD1 4HN, Scotland, UK.; School of Science and Technology, Nottingham Trent University, Nottingham, UK.
Jazyk: angličtina
Zdroj: Drug testing and analysis [Drug Test Anal] 2015 Oct; Vol. 7 (10), pp. 926-36. Date of Electronic Publication: 2015 Apr 05.
DOI: 10.1002/dta.1790
Abstrakt: Phenazepam is a benzodiazepine that is predominantly used clinically in the former Soviet states but is being abused throughout the wider world. This study reports the tissue distribution and concentration of both phenazepam and 3-hydroxyphenazepam in 29 cases quantitated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a variety of post-mortem fluids (subclavian blood, femoral blood, cardiac blood, urine, vitreous humour) and tissues (thalamus, liver and psoas muscle). In 27 cases, the cause of death was not directly related to phenazepam (preserved (fluoride/oxalate) femoral blood phenazepam concentrations 0.007 mg/L to 0.360 mg/L (median 0.097 mg/L). In two cases, phenazepam was either a contributing factor to, or the certified cause of death (preserved (fluoride/oxalate) femoral blood 0.97 mg/L and 1.64 mg/L). The analysis of phenazepam and 3-hydroxyphenazepam in this study suggests that they are unlikely to be subject to large post-mortem redistribution and that there is no direct correlation between tissues/fluid and femoral blood concentrations. Preliminary investigations of phenazepam stability comparing femoral blood phenazepam concentrations in paired preserved (2.5% fluoride/oxalate) and unpreserved blood show that unpreserved samples show on average a 14% lower concentration of phenazepam and we recommend that phenazepam quantitation is carried out using preserved samples wherever possible.
(Copyright © 2015 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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