Spatially heterogeneous choroid plexus transcriptomes encode positional identity and contribute to regional CSF production.

Autor:	Lun MP; Department of Pathology, and Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118., Johnson MB; Division of Genetics, Boston Children's Hospital, Boston, Massachusetts 02115., Broadbelt KG; Department of Pathology, and., Watanabe M; Department of Pathology and Laboratory Medicine, University of California Irvine School of Medicine, Irvine, California 92697., Kang YJ; Department of Pathology and Laboratory Medicine, University of California Irvine School of Medicine, Irvine, California 92697., Chau KF; Department of Pathology, and., Springel MW; Department of Pathology, and., Malesz A; Department of Pathology, and., Sousa AM; Department of Neurobiology and Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, Connecticut 06510., Pletikos M; Department of Neurobiology and Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, Connecticut 06510., Adelita T; Department of Pathology, and Department of Biochemistry, Federal University of São Paulo, São Paulo 04039, Brazil, and., Calicchio ML; Department of Pathology, and., Zhang Y; Pulmonary and Critical Care Medicine, Department of Medicine, Washington University, St Louis, Missouri 63110., Holtzman MJ; Pulmonary and Critical Care Medicine, Department of Medicine, Washington University, St Louis, Missouri 63110., Lidov HG; Department of Pathology, and., Sestan N; Department of Neurobiology and Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, Connecticut 06510., Steen H; Department of Pathology, and., Monuki ES; Department of Pathology and Laboratory Medicine, University of California Irvine School of Medicine, Irvine, California 92697., Lehtinen MK; Department of Pathology, and maria.lehtinen@childrens.harvard.edu.
Jazyk:	angličtina
Zdroj:	The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2015 Mar 25; Vol. 35 (12), pp. 4903-16.
DOI:	10.1523/JNEUROSCI.3081-14.2015
Abstrakt:	A sheet of choroid plexus epithelial cells extends into each cerebral ventricle and secretes signaling factors into the CSF. To evaluate whether differences in the CSF proteome across ventricles arise, in part, from regional differences in choroid plexus gene expression, we defined the transcriptome of lateral ventricle (telencephalic) versus fourth ventricle (hindbrain) choroid plexus. We find that positional identities of mouse, macaque, and human choroid plexi derive from gene expression domains that parallel their axial tissues of origin. We then show that molecular heterogeneity between telencephalic and hindbrain choroid plexi contributes to region-specific, age-dependent protein secretion in vitro. Transcriptome analysis of FACS-purified choroid plexus epithelial cells also predicts their cell-type-specific secretome. Spatial domains with distinct protein expression profiles were observed within each choroid plexus. We propose that regional differences between choroid plexi contribute to dynamic signaling gradients across the mammalian cerebroventricular system. (Copyright © 2015 the authors 0270-6474/15/354903-14$15.00/0.)
Databáze:	MEDLINE
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