Anterior cruciate ligament- and hamstring tendon-derived cells: in vitro differential properties of cells involved in ACL reconstruction.

Autor: Ghebes CA; MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands., Kelder C; MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands., Schot T; MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands., Renard AJ; Department of Orthopaedic Surgery, Medisch Spectrum Twente Hospital, Enschede, The Netherland., Pakvis DF; Department of Orthopaedics and Traumatology, Orthopaedic Centre OCON, Hengelo, The Netherlands., Fernandes H; MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.; Center for Neuroscience and Cell Biology (CNC), Stem Cells and Drug Screening group, University of Coimbra, Coimbra, Portugal., Saris DB; MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.; Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.
Jazyk: angličtina
Zdroj: Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2017 Apr; Vol. 11 (4), pp. 1077-1088. Date of Electronic Publication: 2015 Mar 11.
DOI: 10.1002/term.2009
Abstrakt: Anterior cruciate ligament (ACL) reconstruction involves the replacement of the torn ligament with a new graft, often a hamstring tendon (HT). Described as similar, the ACL and HT have intrinsic differences related to their distinct anatomical locations. From a cellular perspective, identifying these differences represents a step forward in the search for new cues that enhance recovery after the reconstruction. The purpose of this study was to characterize the phenotype and multilineage potential of ACL- and HT-derived cells. ACL- and HT-derived cells were isolated from tissue harvest from patients undergoing total knee arthroplasty (TKA) or ACL reconstruction. In total, three ACL and three HT donors were investigated. Cell morphology, self-renewal potential (CFU-F), surface marker profiling, expression of tendon/ligament-related markers (PCR) and multilineage potential were analysed for both cell types; both had fibroblast-like morphology and low self-renewal potential. No differences in the expression of tendon/ligament-related genes or a selected set of surface markers were observed between the two cell types. However, differences in their multilineage potential were observed: while ACL-derived cells showed a high potential to differentiate into chondrocytes and adipocytes, but not osteoblasts, HT-derived cells showed poor potential to form adipocytes, chondrocytes and osteoblasts. Our results demonstrated that HT-derived cells have low multilineage potential compared to ACL-derived cells, further highlighting the need for extrinsic signals to fully restore the function of the ACL upon reconstruction. Copyright © 2015 John Wiley & Sons, Ltd.
(Copyright © 2015 John Wiley & Sons, Ltd.)
Databáze: MEDLINE