High Final Energy of Low-Level Gallium Arsenide Laser Therapy Enhances Skeletal Muscle Recovery without a Positive Effect on Collagen Remodeling.

Autor: de Freitas CE; Post Graduate Program in General and Applied Biology, Bioscience Institute, State University of São Paulo, UNESP, Botucatu, SP, Brazil.; Department of Physiotherapy, University of Oeste Paulista (UNOESTE), Presidente Prudente, SP, Brazil., Bertaglia RS; Post Graduate Program in General and Applied Biology, Bioscience Institute, State University of São Paulo, UNESP, Botucatu, SP, Brazil., Vechetti Júnior IJ; Post Graduate Program in General and Applied Biology, Bioscience Institute, State University of São Paulo, UNESP, Botucatu, SP, Brazil., Mareco EA; Post Graduate Program in General and Applied Biology, Bioscience Institute, State University of São Paulo, UNESP, Botucatu, SP, Brazil., Salomão RA; Post Graduate Program in General and Applied Biology, Bioscience Institute, State University of São Paulo, UNESP, Botucatu, SP, Brazil., de Paula TG; Post Graduate Program in General and Applied Biology, Bioscience Institute, State University of São Paulo, UNESP, Botucatu, SP, Brazil., Nai GA; Department of Pathology, University of Oeste Paulista (UNOESTE), Presidente Prudente, SP, Brazil., Carvalho RF; Department of Morphology, Bioscience Institute State University of São Paulo, UNESP, Botucatu, SP, Brazil., Pacagnelli FL; Department of Physiotherapy, University of Oeste Paulista (UNOESTE), Presidente Prudente, SP, Brazil., Dal-Pai-Silva M; Department of Morphology, Bioscience Institute State University of São Paulo, UNESP, Botucatu, SP, Brazil.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2015 Jul-Aug; Vol. 91 (4), pp. 957-65. Date of Electronic Publication: 2015 Mar 28.
DOI: 10.1111/php.12446
Abstrakt: The aim of this study was to evaluate the effects of a Gallium Arsenide (GaAs) laser, using a high final energy of 4.8 J, during muscle regeneration after cryoinjury. Thirty Wistar rats were divided into three groups: Control (C, n = 10); Injured (I, n = 10) and Injured and laser treated (Injured/LLLT, n = 10). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The applications of the laser (904 nm, 50 mW average power) were initiated 24 h after injury, at energy density of 69 J cm(-1) for 48 s, for 5 days, to two points of the lesion. Twenty-four hours after the final application, the TA muscle was removed and frozen in liquid nitrogen to assess the general muscle morphology and the gene expression of TNF-α, TGF-β, MyoD, and Myogenin. The Injured/LLLT group presented a higher number of regenerating fibers and fewer degenerating fibers (P < 0.05) without changes in the collagen remodeling. In addition, the Injured/LLLT group presented a significant decrease in the expression of TNF-α and myogenin compared to the injured group (P < 0.05). The results suggest that the GaAs laser, using a high final energy after cryoinjury, promotes muscle recovery without changing the collagen remodeling in the muscle extracellular matrix.
(© 2015 The American Society of Photobiology.)
Databáze: MEDLINE
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