Effects of electroacupuncture on experimental periodontitis in rats.

Autor: Lisboa MR; Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil., Gondim DV; †Department of Morphology, Faculty of Medicine, Federal University of Ceará., Ervolino E; ‡Department of Basic Sciences, Division of Histology, Dental School of Aracatuba, São Paulo State University, Aracatuba, São Paulo, Brazil., Vale ML; §Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará., Frota NP; ‖Department of Oral and Maxillofacial Surgery and Periodontology, Ribeirao Preto School of Dentistry, University of São Paulo, Ribeirao Preto, São Paulo, Brazil., Nunes NL; Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil., Mariguela VC; ¶Department of Veterinary Pathology, São Paulo State University, Jaboticabal, São Paulo, Brazil., Taba M Jr; ‖Department of Oral and Maxillofacial Surgery and Periodontology, Ribeirao Preto School of Dentistry, University of São Paulo, Ribeirao Preto, São Paulo, Brazil., Messora MR; ‖Department of Oral and Maxillofacial Surgery and Periodontology, Ribeirao Preto School of Dentistry, University of São Paulo, Ribeirao Preto, São Paulo, Brazil., Furlaneto FA; ‖Department of Oral and Maxillofacial Surgery and Periodontology, Ribeirao Preto School of Dentistry, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of periodontology [J Periodontol] 2015 Jun; Vol. 86 (6), pp. 801-11. Date of Electronic Publication: 2015 Mar 05.
DOI: 10.1902/jop.2015.140630
Abstrakt: Background: Acupuncture has shown the capability of modulating the immuno-inflammatory response of the host. This study aims to evaluate the effects of electroacupuncture (EA) on ligature-induced periodontitis in rats.
Methods: Thirty-two animals were divided into four groups: 1) control; 2) experimental periodontitis (EP); 3) sham-treated (EP/EA-sham); and 4) treated with EA (EP/EA). For the EP groups, a ligature was placed around the right mandibular first molars at day 1. Sessions of EA or EA-sham were assigned every other day. For EA treatment, large intestine meridian points LI4 and LI11 and stomach meridian points ST36 and ST44 were used. EA-sham was performed in off-meridian points. Animals were euthanized at day 11. Histomorphometric and microtomographic analyses were performed. Immunolabeling patterns for the receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and tartrate-resistant acid phosphatase (TRAP) were assessed. Expressions of interleukin (IL)-1β, matrix metalloproteinase (MMP)-8, IL-6, and cyclooxygenase (COX)-2 messenger RNAs (mRNAs) were evaluated by quantitative reverse transcription-polymerase chain reaction. Data were analyzed statistically (P <0.05, analysis of variance).
Results: Histomorphometric and microtomographic analyses demonstrated that group EP/EA presented reduced alveolar bone loss when compared to group EP (P <0.05). Reduced RANKL immunolabeling and fewer TRAP-positive multinucleated cells were observed in the EA-treated group in relation to group EP. No differences were observed in OPG expression among groups. EA treatment decreased the genic expression of IL-1β and MMP-8 (P <0.05), increased the mRNA expression of IL-6 (P <0.05), and did not modify the genic expression of COX-2 in animals with EP (P >0.05).
Conclusion: It can be concluded that EA reduced periodontal tissue breakdown and the expression of some proinflammatory mediators and a proresorptive factor in EP in rats.
Databáze: MEDLINE
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