Role of endogenous PDGF-BB in cultured cardiomyocytes exposed to hypoxia.

Autor: Rong R; Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China; Cadre's Ward, Anhui Provincial Hospital, Hefei 230001, Anhui, China., Wang YC; Institute of Neurological Disease, State Key Lab of Biotherapy, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China., Hu LQ; Cadre's Ward, Anhui Provincial Hospital, Hefei 230001, Anhui, China., He QQ; Institute of Neurological Disease, State Key Lab of Biotherapy, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China., Zhou XF; School of Pharmacy and Medical Sciences, Sansom Institute, Division of Health Sciences, University of South Australia, Adelaide, SA, Australia., Wang TH; Institute of Neuroscience, Kunming Medical University, Kunming, 650031, China; Institute of Neurological Disease, State Key Lab of Biotherapy, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China., Bu PL; Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China. Electronic address: bupeili@medmail.com.cn.
Jazyk: angličtina
Zdroj: Neuropeptides [Neuropeptides] 2015 Apr; Vol. 50, pp. 43-9. Date of Electronic Publication: 2014 Dec 08.
DOI: 10.1016/j.npep.2014.12.001
Abstrakt: Platelet-derived growth factor-BB (PDGF-BB) plays a critical role in cell proliferation, angiogenesis and fibrosis. However, its exact role in cardiomyocytes exposed to hypoxia is not well known. This study was therefore designed to detect whether PDGF-BB expression was changed in a hypoxic condition, then the possible role of endogenous PDGF-BB in cardiomyocytes was explored, with interference RNA in a lentiviral vector ex vivo. The results showed that cultured cardiomyocytes exhibited an optimal proliferation from 3 to 10 days. However, LDH level was significantly increased but the heart rhythm was not altered in cardiomyocytes exposed to hypoxia for 24 hours. PDGF-BB expression was substantially upregulated in hypoxic cardiomyocytes. In order to know the role of PDGF-BB, we performed PDGF-BB knockdown in cultured cardiomyocytes. The number of apoptotic cells and the level of LDH were significantly increased but the beat rhythm was reduced in cardiomyocytes with PDGF-BB knockdown. These findings suggest that endogenous PDGF-BB exerts a crucial protective effect to cultured cardiomyocytes exposed to hypoxia.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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