Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts.

Autor: Velickovic M; Center for Molecular Medicine, Kragujevac, Serbia., Pejnovic N; Center for Molecular Medicine, Kragujevac, Serbia., Petrovic R; Department of Endodontics, Faculty of Stomatology, University of Belgrade, Belgrade, Serbia., Mitrovic S; Center for Molecular Medicine, Kragujevac, Serbia., Jeftic I; Center for Molecular Medicine, Kragujevac, Serbia., Kanjevac T; Department of Preventive and Pediatric Dentistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia., Lukic A; Department of Endodontics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Jazyk: angličtina
Zdroj: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology [J Oral Pathol Med] 2016 Jan; Vol. 45 (1), pp. 70-6. Date of Electronic Publication: 2015 Feb 11.
DOI: 10.1111/jop.12312
Abstrakt: Background: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions.
Methods: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression.
Results: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P < 0.05), while the numbers of IL-33- and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33- and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells.
Conclusions: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33- and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.
(© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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