| Autor: |
Forkink M; Department of Biochemistry, Raboud Institute for Molecular Life Sciences, Radboud University Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands., Willems PH, Koopman WJ, Grefte S |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1264, pp. 161-9. |
| DOI: |
10.1007/978-1-4939-2257-4_15 |
| Abstrakt: |
Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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