| Autor: |
Serim S; Lehrstuhl für Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany. steven.verhelst@isas.de., Baer P, Verhelst SH |
| Jazyk: |
angličtina |
| Zdroj: |
Organic & biomolecular chemistry [Org Biomol Chem] 2015 Feb 28; Vol. 13 (8), pp. 2293-9. |
| DOI: |
10.1039/c4ob02444c |
| Abstrakt: |
Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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