Electroacupuncture ameliorates experimental colitis induced by TNBS through activation of interleukin-10 and inhibition of iNOS in mice.
| Autor: | Goes AC; Department of Surgery, Federal University of Ceara, Fortaleza, CE, Brazil., Pinto FM; School of Pharmacy, UFC, Fortaleza, CE, Brazil., Fernandes GC; School of Pharmacy, UFC, Fortaleza, CE, Brazil., Barbosa JS; School of Medicine, UFC, Fortaleza, CE, Brazil., Correia ES; Walter Cantidio Hospital, UFC, Fortaleza, CE, Brazil., Ribeiro RA; Department of Surgery, UFC, Fortaleza, CE, Brazil., Guimaraes SB; UFC, Fortaleza, CE, Brazil., Lima Júnior RC; Department of Pharmacology, UFC, Fortaleza, CE, Brazil., Brito GA; Department of Morphology, UFC, Fortaleza, CE, Brazil., Rodrigues LV; Department of Surgery, UFC, Fortaleza, CE, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Acta cirurgica brasileira [Acta Cir Bras] 2014 Dec; Vol. 29 (12), pp. 787-93. |
| DOI: | 10.1590/S0102-86502014001900004 |
| Abstrakt: | Purpose: To study the anti-inflammatory actions of electroacupuncture (EAc) on an experimental colitis model in mice. Methods: Thirty-eight male Swiss mice, divided in five groups, were subjected to induction of colitis by TNBS in 50% ethanol. Saline (SAL) and ethanol (ETNL) groups served as controls. TNBS+EAc and TNBS+ dexamethasone subgroups were treated with EAc 100Hz and dexamethasone (DEXA) 1 mg/Kg/day, respectively. After three days, a colon segment was obtained for quantification of myeloperoxidase (MPO) activity, immunohistochemistry for iNOS, malondialdehyde (MDA) and cytokines (IL-1β and IL-10). Results: Neutrophilic activity, assayed as MPO activity, was significantly higher in the TNBS colitis group than that in the saline control group. TNBS+EAc group showed suppression of IL-10 in the colon. EAc treatment significantly reduced the concentration of MDA and the expression of iNOS, as compared to the other groups. Conclusion: Electroacupuncture 100Hz applied to acupoint ST-36 promotes an anti-inflammatory action on the TNBS-induced colitis, mediated by increase of IL-10 and decrease of iNOS expression. |
| Databáze: | MEDLINE |
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