Elastin-like recombinamer-covered stents: Towards a fully biocompatible and non-thrombogenic device for cardiovascular diseases.

Autor: de Torre IG; BIOFORGE, CIBER-BBN, Campus 'Miguel Delibes' Centro I+D, Universidad de Valladolid, Paseo Belén 11, 47011 Valladolid, Spain. Electronic address: igonzalez@bioforge.uva.es., Wolf F; Tissue Engineering and Textile Implants, AME, Helmholtz Institute, RWTH, Aachen, Germany., Santos M; BIOFORGE, CIBER-BBN, Campus 'Miguel Delibes' Centro I+D, Universidad de Valladolid, Paseo Belén 11, 47011 Valladolid, Spain., Rongen L; Tissue Engineering and Textile Implants, AME, Helmholtz Institute, RWTH, Aachen, Germany., Alonso M; BIOFORGE, CIBER-BBN, Campus 'Miguel Delibes' Centro I+D, Universidad de Valladolid, Paseo Belén 11, 47011 Valladolid, Spain., Jockenhoevel S; Tissue Engineering and Textile Implants, AME, Helmholtz Institute, RWTH, Aachen, Germany; Institut für Textiltechnik, RWTH Aachen University, Aachen, Germany., Rodríguez-Cabello JC; BIOFORGE, CIBER-BBN, Campus 'Miguel Delibes' Centro I+D, Universidad de Valladolid, Paseo Belén 11, 47011 Valladolid, Spain., Mela P; Tissue Engineering and Textile Implants, AME, Helmholtz Institute, RWTH, Aachen, Germany.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2015 Jan; Vol. 12, pp. 146-155. Date of Electronic Publication: 2014 Oct 29.
DOI: 10.1016/j.actbio.2014.10.029
Abstrakt: We explored the use of recently developed gels obtained by the catalyst free click reaction of elastin-like recombinamers (ELRs) to fabricate a new class of covered stents. The approach consists in embedding bare metal stents in the ELR gels by injection molding, followed by endothelialization under dynamic pressure and flow conditions in a bioreactor. The mechanical properties of the gels could be easily tuned by choosing the adequate concentration of the ELR components and their biofunctionality could be tailored by inserting specific sequences (RGD and REDV). The ELR-covered stents exhibited mechanical stability under high flow conditions and could undergo crimping and deployment without damage. The presence of RGD in the ELR used to cover the stent supported full endothelialization in less than 2weeks in vitro. Minimal platelet adhesion and fibrin adsorption were detected after exposure to blood, as shown by immunostaining and scanning electron microscopy. These results prove the potential of this approach towards a new and more effective generation of covered stents which exclude the atherosclerotic plaque from the blood stream and have high biocompatibility, physiological hemocompatibility and reduced response of the immune system.
(Copyright © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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