Analysis of heparanase isoforms and cathepsin B in the plasma of patients with gastrointestinal carcinomas: analytical cross-sectional study.

Autor:	Melo CM; Department of Biochemistry, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil., Origassa CS; Department of Biochemistry, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil., Theodoro TR; Faculdade de Medicina do ABC (FMABC), Santo André, São Paulo, Brazil., Matos LL; Department of Biochemistry, Faculdade de Medicina do ABC (FMABC), Santo André, São Paulo, Brazil., Miranda TA; Department of Biochemistry, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil., Accardo CM; Department of Biochemistry, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil., Bouças RI; Department of Biochemistry, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil., Suarez ER; Department of Biochemistry, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil., Pares MM; Associação Beneficente de Coleta de Sangue (Colsan), São Paulo, São Paulo, Brazil., Waisberg DR; Surgery Department, Universidade de São Paulo (USP), São Paulo, Brazil., Toloi GC; Faculdade de Medicina do ABC (FMABC), Santo André, São Paulo, Brazil., Nader HB; Department of Biochemistry, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil., Waisberg J; Department of Surgery, Faculdade de Medicina do ABC (FMABC), Santo André, São Paulo, Brazil., Pinhal MA; Department of Biochemistry, Faculdade de Medicina do ABC (FMABC), Santo André, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Sao Paulo medical journal = Revista paulista de medicina [Sao Paulo Med J] 2015 Feb; Vol. 133 (1), pp. 28-35.
DOI:	10.1590/1516-3180.2013.7080003
Abstrakt:	Context and Objective: Heparanase-1 degrades heparan sulfate and has been correlated with tumor progression. Although the isoform heparanase-2 has no catalytic activity, it seems to be important for modulating heparanase-1 activity. Cathepsin B is a proteinase involved in tumor metastasis. The aim of this study was to analyze heparanase isoform expression and cathepsin B activity in plasma samples from patients with gastrointestinal carcinomas, compared with healthy individuals (control group). Design and Setting: This was an analytical cross-sectional study. Peripheral blood samples were collected at a Brazilian public hospital, from 21 patients with histopathological diagnoses of gastrointestinal carcinomas and from 43 healthy individuals. The analyses were performed in two Brazilian medical schools. Methods: Heparanase isoforms were identified and quantified in plasma samples by means of Western blot. The enzymatic activities of heparanase-1 and cathepsin B were also measured. Results: The results demonstrated that the expression of both heparanase isoforms was significantly greater in plasma samples from gastrointestinal carcinoma patients, compared with the control group. Logistic regression analysis showed that increased heparanase-1 and heparanase-2 expression was exclusively dependent on the tumor. There was a significant increase in heparanase-1 and cathepsin B activity in the patients' plasma. Conclusion: Overexpression of heparanase-1 and heparanase-2, along with increased heparanase-1 and cathepsin B activity in plasma, is associated with the diagnosis of gastrointestinal carcinoma. These findings provide support for using non-invasive assays (plasma samples) as an auxiliary method for diagnosing gastrointestinal tumors.
Databáze:	MEDLINE
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