| Autor: |
Martínez CR; Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. claudio@biocen.cu., Rodríguez TL; Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. lobaina@biocen.cu., Zhurbenko R; Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. raisa@biocen.cu., Valdés IA; Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. ivonne.alfonso@biocen.cu., Gontijo SM; Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. savio.morato@yahoo.com.br., Gomes AD; Chemistry Department, ICEx, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. alinnedamasia@gmail.com., Suarez DF; Chemistry Department, ICEx, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. diego.fersuar@gmail.com., Sinisterra RD; Chemistry Department, ICEx, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. sinisterra@ufmg.br., Cortés ME; Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. mecortes@ufmg.br. |
| Abstrakt: |
Current procedures for the detection and identification of bacterial infections are laborious, time-consuming, and require a high workload and well-equipped laboratories. Therefore the work presented herein developed a simple, fast, and low cost method for bacterial detection based on hydroxyapatite nanoparticles with a nutritive mixture and the fluorogenic substrate. Calcium phosphate ceramic nanoparticles were characterized and integrated with a nutritive mixture for the early detection of bacteria by visual as well as fluorescence spectroscopy techniques. The composite was obtained by combining calcium phosphate nanoparticles (Ca:P ratio, 1.33:1) with a nutritive mixture of protein hydrolysates and carbon sources, which promote fast bacterial multiplication, and the fluorogenic substrate 4-methylumbellipheryl-β-D-glucuronide (MUG). The composite had an average particle size of 173.2 nm and did not show antibacterial activity against Gram-negative or Gram-positive bacteria. After an Escherichia coli suspension was in contact with the composite for 60-90 min, fluorescence detected under UV light or by fluorescence spectrophotometer indicated the presence of bacteria. Intense fluorescence was observed after incubation for a maximum of 90 min. Thus, this calcium phosphate nanocomposite system may be useful as a model for the development of other nanoparticle composites for detection of early bacterial adhesion. |
