Late biliary obstruction in Wistar rats after intermittent hepatic pedicle clamping.

Autor: Jorge Gd; Laboratory of Experimental Liver Transplantation; Brazil., Tártaro Rd; Laboratory of Experimental Liver Transplantation; Brazil., Facin AC; Experimental Medicine and Surgery Center (EMSC); Brazil., Pereira RA; Experimental Medicine and Surgery Center (EMSC); Brazil., Escanhoela CA; Department of Pathology; Brazil., Boin IF; Unit of Liver Transplantation, State University of Campinas, Brazil. Electronic address: ilkaboin@yahoo.com.
Jazyk: angličtina
Zdroj: Transplantation proceedings [Transplant Proc] 2014 Jul-Aug; Vol. 46 (6), pp. 1875-8.
DOI: 10.1016/j.transproceed.2014.05.067
Abstrakt: Introduction: The temporary vascular occlusion of hepatic flow is one of the essential procedures in hepatic surgery.
Aim: Evaluate the late liver alterations after intermittent pedicle hepatic clamping (IHPC) in Wistar rats.
Methods: Male Wistar rats (n = 14) with average weight of 281.1 g, were anesthetized with intraperitoneal ketamine 5%. The IHPC group (n = 7) was submitted to U-shaped abdominal incision; the hepatic pedicle was isolated and submitted to IHPC ischemia 4 times, 5 minutes each, followed by reperfusion 4 times, 5 minutes each. The simulated operation group (n = 7) was subjected to anesthesia, laparotomy, and manipulation of the hepatic pedicle. On day 35, after fasting for 12 hours, liver biopsies were collected and blood was tested for liver aminotransferases (aspartate aminotransferase/alanine aminotransferase).
Results: All the IHPC group animals had a dilated common bile duct and increased liver enzymes (P < .05 by Mann-Whitney test). Ductular proliferation (100% of cases), porta-porta septa (42.8%), formation of lumps (42.8%), foci of necrosis (14.2%), and bile plugs (14, 2%) were observed only in the IHPC group.
Conclusion: In rats, IHPC caused morphologic features leading to biliary obstruction.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: