An arsenic-specific biosensor with genetically engineered Shewanella oneidensis in a bioelectrochemical system.

Autor: Webster DP; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA., TerAvest MA; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA., Doud DF; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA., Chakravorty A; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA., Holmes EC; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA., Radens CM; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA., Sureka S; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA., Gralnick JA; BioTechnology Institute and Department of Microbiology, University of Minnesota-Twin Cities, St. Paul, MN 55108, USA., Angenent LT; Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA. Electronic address: la249@cornell.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2014 Dec 15; Vol. 62, pp. 320-4. Date of Electronic Publication: 2014 Jul 08.
DOI: 10.1016/j.bios.2014.07.003
Abstrakt: Genetically engineered microbial biosensors have yet to realize commercial success in environmental applications due, in part, to difficulties associated with transducing and transmitting traditional bioluminescent information. Bioelectrochemical systems (BESs) output a direct electric signal that can be incorporated into devices for remote environmental monitoring. Here, we describe a BES-based biosensor with genetically encoded specificity for a toxic metal. By placing an essential component of the metal reduction (Mtr) pathway of Shewanella oneidensis under the control of an arsenic-sensitive promoter, we have genetically engineered a strain that produces increased current in response to arsenic when inoculated into a BES. Our BES-based biosensor has a detection limit of ~40 μM arsenite with a linear range up to 100 μM arsenite. Because our transcriptional circuit relies on the activation of a single promoter, similar sensing systems may be developed to detect other analytes by the swap of a single genetic part.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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