RUNX1T1/MTG8/ETO gene expression status in human t(8;21)(q22;q22)-positive acute myeloid leukemia cells.

Autor: Migas AA; Laboratory of the Genetic Biotechnology, Department of Research, Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, Minsk, Belarus., Mishkova OA; Laboratory of the Genetic Biotechnology, Department of Research, Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, Minsk, Belarus., Ramanouskaya TV; Department of Genetics, Faculty of Biology, Belarusian State University, Minsk, Belarus., Ilyushonak IM; Department of Genetics, Faculty of Biology, Belarusian State University, Minsk, Belarus., Aleinikova OV; Laboratory of the Genetic Biotechnology, Department of Research, Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, Minsk, Belarus., Grinev VV; Department of Genetics, Faculty of Biology, Belarusian State University, Minsk, Belarus. Electronic address: grinev_vv@bsu.by.
Jazyk: angličtina
Zdroj: Leukemia research [Leuk Res] 2014 Sep; Vol. 38 (9), pp. 1102-10. Date of Electronic Publication: 2014 Jun 13.
DOI: 10.1016/j.leukres.2014.06.002
Abstrakt: The RUNX1-RUNX1T1 fusion gene, a product of the nonhomologous balanced translocation t(8;21)(q22;q22), is a complex genetic locus. We performed extensive bioinformatic analysis of transcription initiation as well as transcription termination sites in this locus and predicted a number of different RUNX1T1 transcripts. To confirm and quantify the RUNX1T1 gene expression, we analyzed samples from seven acute myeloid leukemia (AML) patients and from the Kasumi-1 cell line. We found variable activity of the four predicted RUNX1T1 promoters located downstream of the chromosome breakpoint. Nineteen alternative RUNX1T1 transcripts were identified by sequencing at least seventeen of which predictably can be translated into functional proteins. While the RUNX1T1 gene is not expressed in normal hematopoietic cells, it may participate in t(8;21)(q22;q22)-dependent leukemic transformation due to its multiple interactions in cell regulatory network particularly through synergistic or antagonistic effects in relation to activity of RUNX1-RUNX1T1 fusion gene.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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