Lipidomics identifies a requirement for peroxisomal function during influenza virus replication.

Autor:	Tanner LB; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456 NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, Singapore 117456., Chng C; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456., Guan XL; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456 Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, 4002 Basel, Switzerland., Lei Z; Centre for Computational Biology, Duke-NUS Graduate Medical School, Singapore 169857 Stem Cell Biology Program, Duke-NUS Graduate Medical School, Singapore 169857 Research Resources Center, University of Illinois at Chicago, Chicago, IL 60612., Rozen SG; Centre for Computational Biology, Duke-NUS Graduate Medical School, Singapore 169857 Stem Cell Biology Program, Duke-NUS Graduate Medical School, Singapore 169857., Wenk MR; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456 NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, Singapore 117456 Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, 4002 Basel, Switzerland Department of Biological Sciences, National University of Singapore, Singapore 117597.
Jazyk:	angličtina
Zdroj:	Journal of lipid research [J Lipid Res] 2014 Jul; Vol. 55 (7), pp. 1357-65. Date of Electronic Publication: 2014 May 27.
DOI:	10.1194/jlr.M049148
Abstrakt:	Influenza virus acquires a host-derived lipid envelope during budding, yet a convergent view on the role of host lipid metabolism during infection is lacking. Using a mass spectrometry-based lipidomics approach, we provide a systems-scale perspective on membrane lipid dynamics of infected human lung epithelial cells and purified influenza virions. We reveal enrichment of the minor peroxisome-derived ether-linked phosphatidylcholines relative to bulk ester-linked phosphatidylcholines in virions as a unique pathogenicity-dependent signature for influenza not found in other enveloped viruses. Strikingly, pharmacological and genetic interference with peroxisomal and ether lipid metabolism impaired influenza virus production. Further integration of our lipidomics results with published genomics and proteomics data corroborated altered peroxisomal lipid metabolism as a hallmark of influenza virus infection in vitro and in vivo. Influenza virus may therefore tailor peroxisomal and particularly ether lipid metabolism for efficient replication. (Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc.)
Databáze:	MEDLINE
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