The vitamin E-binding protein afamin increases in maternal serum during pregnancy.

Autor: Hubalek M; Department of Gynecology and Obstetrics, Innsbruck Medical University, Innsbruck, Austria., Buchner H; Institute of Medical Informatics, Biometry and Epidemiology, Medical School, Ludwig Maximilian University of Munich, Germany., Mörtl MG; Department of Gynecology and Obstetrics, Medical University of Graz, Austria., Schlembach D; Department of Gynecology and Obstetrics, Medical University of Graz, Austria; Department of Gynecology and Obstetrics, Jena University, Jena, Germany., Huppertz B; Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Austria., Firulovic B; Department of Gynecology and Obstetrics, Innsbruck Medical University, Innsbruck, Austria., Köhler W; Department of Laboratory Medicine, Konventhospital Barmherzige Brüder, Linz, Austria., Hafner E; Department of Obstetrics and Gynecology, Danube Hospital/SMZ-Ost, Vienna, Austria., Dieplinger B; Department of Laboratory Medicine, Konventhospital Barmherzige Brüder, Linz, Austria., Wildt L; Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria., Dieplinger H; Division of Genetic Epidemiology, Department of Medical Genetics, Clinical and Molecular Pharmacology, Innsbruck Medical University, Innsbruck, Austria; Vitateq Biotechnology GmbH, Innsbruck, Austria. Electronic address: hans.dieplinger@i-med.ac.at.
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2014 Jul 01; Vol. 434, pp. 41-7. Date of Electronic Publication: 2014 Apr 24.
DOI: 10.1016/j.cca.2014.03.036
Abstrakt: Background: Afamin is a liver-derived plasma glycoprotein with vitamin E-binding properties and a putative function in fertility. This study evaluated serum afamin concentrations during and postpartum to uncomplicated pregnancies and investigated a potential association between afamin concentrations and pregnancy outcome.
Methods: Afamin serum concentrations were measured in women with uncomplicated pregnancies in a retrospective cohort (n=466) at different gestational ages and a prospective observational study (n=76) in the first, second and third trimester. Furthermore, afamin was determined in the first trimester in a cross-sectional pilot study including women with preeclampsia (PE), pregnancy-induced hypertension (PIH) and women without pregnancy complications (n=13 each). Finally, expression of afamin was investigated in human placental tissue by RT-PCR and immunohistochemistry.
Results: Afamin concentrations increased linearly almost two-fold during pregnancy in both retrospective and prospective studies in women without pregnancy complications with median afamin serum concentrations of 61.9 mg/l, 79.6 mg/l, and 98.6 mg/l in the first, second, and third trimester, respectively. After delivery, median afamin concentrations decreased to baseline values of 54.6 mg/l. In the pilot study with pregnancy complications, women with PE displayed significantly higher median afamin concentrations than did women with uncomplicated pregnancy (70.0 mg/l vs. 55.4 mg/l, P=0.007). Expression analyses revealed no placental afamin expression at either mRNA or protein level in uncomplicated pregnancy.
Conclusion: A linear increase in the maternally expressed glycoprotein afamin during pregnancy may serve as basic reference for subsequent investigations of afamin in pregnancy-related disorders.
(Copyright © 2014. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: