Regulatory effects of IL-18 on cytokine profiles and development of myocarditis during Trypanosoma cruzi infection.

Autor: Esper L; Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil; Postgraduate Program in Health Sciences: Infectious Diseases and Tropical Medicine, Medical School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Utsch L; Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil., Soriani FM; Department of General Biology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil., Brant F; Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil; Postgraduate Program in Health Sciences: Infectious Diseases and Tropical Medicine, Medical School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Esteves Arantes RM; Department of Pathology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil., Campos CF; Department of Pathology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil., Pinho V; Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil., Souza DG; Department of Microbiology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil., Teixeira MM; Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil; Postgraduate Program in Health Sciences: Infectious Diseases and Tropical Medicine, Medical School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Tanowitz HB; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA; Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA., Vieira LQ; Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil; Postgraduate Program in Biological Science, NUPEB, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Machado FS; Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, MG, Brazil; Postgraduate Program in Health Sciences: Infectious Diseases and Tropical Medicine, Medical School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: machadofs@gmail.com.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2014 Jun; Vol. 16 (6), pp. 481-90. Date of Electronic Publication: 2014 Apr 01.
DOI: 10.1016/j.micinf.2014.03.007
Abstrakt: Chagas disease, caused by Trypanosoma cruzi (Tc), is an important cause of heart disease. Resistance to Tc infection is multifactorial and associated with Th1 response. IL-18 plays an important role in regulation of IFN-γ production/development of Th1 response. However, the role of IL-18 in the setting of Tc infection remains unclear. Therefore, we investigated the role of IL-18 in the modulation of immune response and myocarditis in Tc infection. C57BL/6 and IL-18 KO mice were infected with Tc (Y or Colombian strain) and parasitemia, immune response and pathology were evaluated. Y strain infection of IL-18 KO did not alter any parameters when compared with C57BL/6 mice. However, during the acute phase (20 and 40 days post infection-dpi), Colombian strain infected-IL-18 KO mice displayed higher serum levels of IL-12 and IFN-γ, respectively, and at the chronic phase (100 dpi) an increase in splenic IFN-γ-producing CD4(+) and CD8(+) T memory cells. There was an IL-10, FOXP3 and CD4(+)CD25(+) cells reduction during acute infection in spleen. Additionally, there was a significant reduction in leukocyte infiltration and parasite load in myocardium of chronically infected IL-18 KO mice. Collectively, these data indicate that IL-18 contributes to the pathogenesis of Tc-induced myocarditis when infected with Colombian but not Y strain. These observations also underscore that parasite and host strain differences are important in evaluation of experimental Tc infection pathogenesis.
(Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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