Recent discoveries and applications involving small-molecule microarrays.

Autor: Hong JA; Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Neel DV; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Wassaf D; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Caballero F; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Koehler AN; Broad Institute of MIT and Harvard, Cambridge, MA, USA; David H. Koch Institute for Integrative Cancer Research, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: koehler@mit.edu.
Jazyk: angličtina
Zdroj: Current opinion in chemical biology [Curr Opin Chem Biol] 2014 Feb; Vol. 18, pp. 21-8. Date of Electronic Publication: 2013 Nov 27.
DOI: 10.1016/j.cbpa.2013.09.020
Abstrakt: High-throughput and unbiased binding assays have proven useful in probe discovery for a myriad of biomolecules, including targets of unknown structure or function and historically challenging target classes. Over the past decade, a number of novel formats for executing large-scale binding assays have been developed and used successfully in probe discovery campaigns. Here we review the use of one such format, the small-molecule microarray (SMM), as a tool for discovering protein-small molecule interactions. This review will briefly highlight selected recent probe discoveries using SMMs as well as novel uses of SMMs in profiling applications.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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