Nicotinamide adenine dinucleotide phosphate oxidase is differentially regulated in normal myometrium versus leiomyoma.

Autor: Fletcher NM; Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA., Saed MG; Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA., Abuanzeh S; Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA., Abu-Soud HM; Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA., Al-Hendy A; Department of Obstetrics and Gynecology, Meharry Medical College, Nashville, TN, USA., Diamond MP; Department of Obstetrics and Gynecology, Georgia Regents University, Augusta, GA, USA., Saed GM; Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA gsaed@med.wayne.edu.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2014 Sep; Vol. 21 (9), pp. 1145-52. Date of Electronic Publication: 2014 Feb 11.
DOI: 10.1177/1933719114522552
Abstrakt: Uterine fibroids are the most common benign tumor in women. The goal of this study was to investigate whether nicotinamide adenine dinucleotide phosphate oxidase (NOX), a major source of superoxide and subsequent oxidative stress, was differentially regulated in myometrium versus leiomyoma. Expression levels of NOXs1-5, dual oxidase (DUOX), DUOX2, NOX organizer (NOXO) 1, NOX activator 1, p47(phox), p67(phox), and p22(phox) were determined in cells treated with hypoxia by real-time reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry in tissues. Expression of NOX4 increased in fibroid compared to myometrial tissues and cells. The NOX2, DUOX1, and p67(phox) were higher while p22(phox) was lower in fibroid than that in myometrial cells. Hypoxia increased NOX4, DUOX1, and NOXO1 and decreased p22(phox) in myometrial and reduced DUOX1 in fibroid cells. The NOX1, NOX3, NOX5, and DUOX2 were undetectable. Fibroid cells are characterized by a unique NOX profile, which promotes a severe prooxidant state that may be responsible for their development. Targeting these subunits may be beneficial for future therapeutic interventions.
(© The Author(s) 2014.)
Databáze: MEDLINE
Externí odkaz: