Multicenter evaluation of the Sepsityper™ extraction kit and MALDI-TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK(®) diagnostic blood culture systems.

Autor: Schieffer KM; The Johns Hopkins Hospital, Baltimore, MD, USA., Tan KE, Stamper PD, Somogyi A, Andrea SB, Wakefield T, Romagnoli M, Chapin KC, Wolk DM, Carroll KC
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2014 Apr; Vol. 116 (4), pp. 934-41. Date of Electronic Publication: 2014 Feb 08.
DOI: 10.1111/jam.12434
Abstrakt: Aims: (i) Evaluation of delayed time to blood culture extraction by the Sepsityper kit and impact of shipping pellets off-site for MALDI-TOF MS analysis. (ii) Comparison of Sepsityper and laboratory-developed extraction methods from a literature review.
Methods and Results: Using two blood culture systems (BD BACTEC and VersaTREK), we extracted 411 positive blood cultures using the Sepsityper kit to mimic a potential protocol for institutions without a MALDI-TOF MS. Extracted pellets were shipped and analysed on the Bruker UltraflexIII. Successful extraction of 358 (87·1%) samples was determined by the presence of detectable proteins. MALDI-TOF MS correctly identified 332 (80·8%) samples.
Conclusions: Delayed time to extraction did not affect Sepsityper extraction or MALDI-TOF MS accuracy. The extracted pellets remain stable and provide accurate results by MALDI-TOF MS when shipped at room temperature to off-site reference laboratories.
Significance and Impact of the Study: This is the first study to show that institutions without a MALDI-TOF MS can take advantage of this innovative technology by shipping a volume of blood to an off-site laboratory for extraction and MALDI-TOF MS analysis. We also performed a literature review to compare various extraction methods.
(© 2014 The Society for Applied Microbiology.)
Databáze: MEDLINE
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