Is L-glutathione more effective than L-glutamine in preventing enteric diabetic neuropathy?

Autor: Hermes-Uliana C; Department of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil., Panizzon CP, Trevizan AR, Sehaber CC, Ramalho FV, Martins HA, Zanoni JN
Jazyk: angličtina
Zdroj: Digestive diseases and sciences [Dig Dis Sci] 2014 May; Vol. 59 (5), pp. 937-48. Date of Electronic Publication: 2013 Dec 27.
DOI: 10.1007/s10620-013-2993-2
Abstrakt: Background: Diabetes and its complications appear to be multifactorial. Substances with antioxidant potential have been used to protect enteric neurons in experimental diabetes.
Aim: This study evaluated the effects of supplementation with L-glutamine and L-glutathione on enteric neurons in the jejunum in diabetic rats.
Methods: Rats at 90 days of age were distributed into six groups: normoglycemic, normoglycemic supplemented with 2 % L-glutamine, normoglycemic supplemented with 1 % L-glutathione, diabetic (D), diabetic supplemented with 2 % L-glutamine (DG), and diabetic supplemented with 1 % L-glutathione (DGT). After 120 days, the jejunums were immunohistochemically stained for HuC/D+ neuronal nitric oxide synthase (nNOS) and vasoactive intestinal polypeptide (VIP). Western blot was performed to evaluate nNOS and VIP. Submucosal and myenteric neurons were quantitatively and morphometrically analyzed.
Results: Diabetic neuropathy was observed in myenteric HuC/D, nNOS, and VIP neurons (p < 0.05). In the submucosal plexus, diabetes did not change nitrergic innervation but increased VIPergic neuronal density and body size (p < 0.05). Supplementation with L-glutathione prevented changes in HuC/D neurons in the enteric plexus (p < 0.05), showing that supplementation with L-glutathione was more effective than with L-glutamine. Myenteric nNOS neurons in the DGT group exhibited a reduced density (34.5 %) and reduced area (p < 0.05). Submucosal neurons did not exhibit changes. The increase in VIP-expressing neurons was prevented in the submucosal plexus in the DG and DGT groups (p < 0.05).
Conclusion: Supplementation with L-glutathione exerted a better neuroprotective effect than L-glutamine and may prevent the development of enteric diabetic neuropathy.
Databáze: MEDLINE
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