| Autor: |
Ingersoll JC; Plant Molecular Biology Laboratory, United States Department of Agriculture, Beltsville Agricultural Research Center, Building 006, 20705-2350, Beltsville, Maryland, USA., Heutte TM, Owens LD |
| Jazyk: |
angličtina |
| Zdroj: |
Plant cell reports [Plant Cell Rep] 1996 Aug; Vol. 15 (11), pp. 836-40. |
| DOI: |
10.1007/BF00233152 |
| Abstrakt: |
Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells. Each construct was expressed in recipient cells at 6 h after bombardment with maximum levels observed between 12 and 48 h. Expression of the PR-S construct mimicked the time-course expression of the constitutively expressed 35S construct but reached levels almost 50% higher. The pin2-promoter construct was ultimately expressed at levels similar to that of PR-S. Expression of the osmotin promoter-leader construct was highest, reaching levels approximately 2.5-fold higher than those of the 35S construct. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink