| Autor: |
Zhou Z; Department of Biology, Miami University , Oxford, OH , USA., Darwal MA, Cheng EA, Taylor SR, Duan E, Harding PA |
| Jazyk: |
angličtina |
| Zdroj: |
Growth factors (Chur, Switzerland) [Growth Factors] 2013 Dec; Vol. 31 (6), pp. 185-98. Date of Electronic Publication: 2013 Oct 14. |
| DOI: |
10.3109/08977194.2013.840297 |
| Abstrakt: |
Abnormal adipogenesis leads to excessive fat accumulation and several health disorders. Mouse fibroblasts (MLC) transfected with ADAM 12S and HB-EGF promoted lipid accumulation. Addition of KBR-7785, an ADAM 12S inhibitor, to HB-EGF/ADAM 12S expressing cells suppressed adipogenesis. BrdU incorporation was attenuated and enhanced mitotracker staining was observed in HB-EGF/ADAM 12S cells. Quantitative real time RT-PCR resulted in elevated levels of expression of three brown adipose tissue (BAT) genes (PRDM16, PGC-1α, and UCP-1), while expression levels of the three white adipose tissue (WAT) genes (PPARγ, C/EBPα, and AKT-1) were unaltered in HB-EGF/ADAM 12S cells. Amino- or carboxy-terminal deletions of HB-EGF (HB-EGFΔN and HB-EGFΔC) co-expressed with ADAM 12S stimulated lipid accumulation. Human epidermoid carcinoma cells (A431) also exhibited lipid accumulation by HB-EGF/ADAM 12S co-expression. These studies suggest ADAM 12S and HB-EGF are involved in cellular plasticity resulting in the production of BAT-like cells and offers insight into novel therapeutic approaches for fighting obesity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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