Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro.

Autor: Yogendra Kumar MS; Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi 110 054, India. msyogendra@gmail.com, Tirpude RJ, Maheshwari DT, Bansal A, Misra K
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2013 Dec 15; Vol. 141 (4), pp. 3443-50. Date of Electronic Publication: 2013 Jun 22.
DOI: 10.1016/j.foodchem.2013.06.057
Abstrakt: Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02 mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551-196.89 mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r(2)=0.99), indicating that reducing ability correlated well with amount of PRF. Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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