Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil.

Autor: Paes Rde C; Agência Estadual de Defesa Sanitária Animal e Vegetal - Iagro/MS, Av. Filinto Muller, 1146, CEP 79074-902, Campo Grande, MS, Brazil. ritinhapaes@yahoo.com.br, Fonseca AA Jr, Monteiro LA, Jardim GC, Piovezan U, Herrera HM, Mauro RA, Vieira-da-Motta O
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2013 Aug 30; Vol. 165 (3-4), pp. 448-54. Date of Electronic Publication: 2013 Apr 09.
DOI: 10.1016/j.vetmic.2013.03.028
Abstrakt: The feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky's disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil.
(Copyright © 2013 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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