Ultrasound-mediated gene transfer (sonoporation) in fibrin-based matrices: potential for use in tissue regeneration.
| Autor: | Nomikou N; Sonidel Ltd, Dublin, Ireland., Feichtinger GA; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Austrian Cluster for Tissue Regeneration, Vienna, Austria., Redl H; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Austrian Cluster for Tissue Regeneration, Vienna, Austria., McHale AP; Department of Pharmacy and Pharmaceutical Sciences, University of Ulster, Northern Ireland. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2016 Jan; Vol. 10 (1), pp. 29-39. Date of Electronic Publication: 2013 Apr 17. |
| DOI: | 10.1002/term.1730 |
| Abstrakt: | It has been suggested that gene transfer into donor cells is an efficient and practical means of locally supplying requisite growth factors for applications in tissue regeneration. Here we describe, for the first time, an ultrasound-mediated system that can non-invasively facilitate gene transfer into cells entrapped within fibrin-based matrices. Since ultrasound-mediated gene transfer is enhanced using microbubbles, we compared the efficacy of neutral and cationic forms of these reagents on the ultrasound-stimulated gene transfer process in gel matrices. In doing so we demonstrated the beneficial effects associated with the use of cationic microbubble preparations that interact directly with cells and nucleic acid within matrices. In some cases, gene expression was increased two-fold in gel matrices when cationic microbubbles were compared with neutral microbubbles. In addition, incorporating collagen into fibrin gels yielded a 25-fold increase in gene expression after application of ultrasound to microbubble-containing matrices. We suggest that this novel system may facilitate non-invasive temporal and spatial control of gene transfer in gel-based matrices for the purposes of tissue regeneration. (Copyright © 2013 John Wiley & Sons, Ltd.) |
| Databáze: | MEDLINE |
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