Label-free cell profiling.

Autor: Schasfoort RB; Medical Cell Biophysics Group, MIRA Institute, University of Twente, Enschede, The Netherlands. r.b.m.schasfoort@utwente.nl, Bentlage AE, Stojanovic I, van der Kooi A, van der Schoot E, Terstappen LW, Vidarsson G
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2013 Aug 01; Vol. 439 (1), pp. 4-6. Date of Electronic Publication: 2013 Apr 11.
DOI: 10.1016/j.ab.2013.04.001
Abstrakt: A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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