| Autor: |
Michaud J; Center for Integrative Genomics, University of Lausanne, Génopode, 1015 Lausanne, Switzerland., Praz V, James Faresse N, Jnbaptiste CK, Tyagi S, Schütz F, Herr W |
| Jazyk: |
angličtina |
| Zdroj: |
Genome research [Genome Res] 2013 Jun; Vol. 23 (6), pp. 907-16. Date of Electronic Publication: 2013 Mar 28. |
| DOI: |
10.1101/gr.150078.112 |
| Abstrakt: |
In human transcriptional regulation, DNA-sequence-specific factors can associate with intermediaries that orchestrate interactions with a diverse set of chromatin-modifying enzymes. One such intermediary is HCFC1 (also known as HCF-1). HCFC1, first identified in herpes simplex virus transcription, has a poorly defined role in cellular transcriptional regulation. We show here that, in HeLa cells, HCFC1 is observed bound to 5400 generally active CpG-island promoters. Examination of the DNA sequences underlying the HCFC1-binding sites revealed three sequence motifs associated with the binding of (1) ZNF143 and THAP11 (also known as Ronin), (2) GABP, and (3) YY1 sequence-specific transcription factors. Subsequent analysis revealed colocalization of HCFC1 with these four transcription factors at ∼90% of the 5400 HCFC1-bound promoters. These studies suggest that a relatively small number of transcription factors play a major role in HeLa-cell transcriptional regulation in association with HCFC1. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
