| Autor: |
Danshina SV; A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow, USSR., Drachev AL, Drachev LA, Eremin SV, Kaulen AD, Khitrina LV, Mitsner BI |
| Jazyk: |
angličtina |
| Zdroj: |
Archives of biochemistry and biophysics [Arch Biochem Biophys] 1990 Jun; Vol. 279 (2), pp. 225-31. |
| DOI: |
10.1016/0003-9861(90)90485-h |
| Abstrakt: |
13-Ethyl-, 13-isopropyl-, 13-tert-butyl-, 13-phenyl-, 13-alpha-naphthyl-, and 13-demethyl-retinals were synthesized and incubated with bacterioopsin (bO) to give the corresponding bacteriorhodopsin (bR) analogs. The capability of the 13-tert-butyl- and 13-alpha-naphthyl-bRs to exist and to photocycle shows that apparently around C(13) of the chromophore there lies a large enough cavity. A study of the light-induced conversions of the artificial pigments prepared has shown that the introduction at position 13 of the chromophore of the hydrocarbon substituents bulkier than that of the natural bR diminished the amplitudes of the electric photoresponses. Bulky C(13)-substituents or absence of substitution at that position decelerated the relaxation of the M-intermediates and disturbed the 13-cis-in equilibrium all-trans-isomerization. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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