Beneficial cardiac effects of caloric restriction are lost with age in a murine model of obesity.

Autor: AlGhatrif M; Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Carnegie 568, Baltimore, MD 21287, USA., Watts VL, Niu X, Halushka M, Miller KL, Vandegaer K, Bedja D, Fox-Talbot K, Bielawska A, Gabrielson KL, Barouch LA
Jazyk: angličtina
Zdroj: Journal of cardiovascular translational research [J Cardiovasc Transl Res] 2013 Jun; Vol. 6 (3), pp. 436-45. Date of Electronic Publication: 2013 Mar 02.
DOI: 10.1007/s12265-013-9453-4
Abstrakt: Obesity is associated with increased diastolic stiffness and myocardial steatosis and dysfunction. The impact of aging on the protective effects of caloric restriction (CR) is not clear. We studied 2-month (younger) and 6-7-month (older)-old ob/ob mice and age-matched C57BL/6J controls (WT). Ob/ob mice were assigned to diet ad libitum or CR for 4 weeks. We performed echocardiograms, myocardial triglyceride assays, Oil Red O staining, and measured free fatty acids, superoxide, NOS activity, ceramide levels, and Western blots. In younger mice, CR restored diastolic function, reversed myocardial steatosis, and upregulated Akt phosphorylation. None of these changes was observed in the older mice; however, CR decreased oxidative stress and normalized NOS activity in these animals. Interestingly, myocardial steatosis was not associated with increased ceramide, but CR altered the composition of ceramides. In this model of obesity, aging attenuates the benefits of CR on myocardial structure and function.
Databáze: MEDLINE
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