Rapid and accurate identification of poliovirus strains used for vaccine production.
| Autor: | Nijst OE; National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven, The Netherlands., Mouthaan JJ, Mekkes DR, Jusic E, van der Avoort HG, Metz B |
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| Jazyk: | angličtina |
| Zdroj: | Journal of virological methods [J Virol Methods] 2013 Apr; Vol. 189 (1), pp. 189-95. Date of Electronic Publication: 2013 Feb 19. |
| DOI: | 10.1016/j.jviromet.2013.01.026 |
| Abstrakt: | In the context of eradication of poliomyelitis the World Health Organization stimulates the development of inactivated polio vaccines based on attenuated virus strains. In addition to vaccine development, tests have to be designed to assess the vaccine quality. An important test is the identification test for poliovirus strains that are used for the vaccine production. A rapid and accurate PCR method with fluorescent probes has been developed to identify unequivocally the vaccine-specific poliovirus strains, such as Mahoney, MEF-1, Saukett H, Sabin type 1, Sabin type 2 and Sabin type 3. (Copyright © 2013 Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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