| Autor: |
Astray RM; Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-900, Brazil, renatoastray@butantan.gov.br., Jorge SA, Lemos MA, Yokomizo AY, Boldorini VL, Puglia AL, Ribeiro OG, Pereira CA |
| Jazyk: |
angličtina |
| Zdroj: |
Cytotechnology [Cytotechnology] 2013 Oct; Vol. 65 (5), pp. 829-38. Date of Electronic Publication: 2013 Jan 23. |
| DOI: |
10.1007/s10616-012-9522-6 |
| Abstrakt: |
Recombinant rabies virus glycoprotein (RVGP) was expressed in cell membranes of stably transfected Drosophila S2 cells using constitutive and inducible promoters. Although with quantitative differences of RVGP expression in both systems, the cDNA transcription, as evaluated by relative RVGP mRNA levels measured by qRT-PCR, sustained the amount of RVGP producing cells and the RVGP volumetric (ΠRVGP) productivity. At the transition to the stationary cell growth phase, once the cell culture slowed down its rate of multiplication, an accumulation of RVGP mRNA and RVGP was clearly observed in both cell populations. Nevertheless, cell cultures performed under sub-optimal temperatures indicated that an envisaged increase in the RVGP production is not only dependent on cell growth rate, but essentially on optimal cell metabolic state. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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