| Autor: |
Ruckhäberle E; Department of Obstetrics and Gynecology, Goethe University Frankfurt, Germany., Karn T, Hanker L, Schwarz J, Schulz-Knappe P, Kuhn K, Böhm G, Selzer S, Erhard N, Engels K, Holtrich U, Kaufmann M, Rody A |
| Jazyk: |
angličtina |
| Zdroj: |
Breast care (Basel, Switzerland) [Breast Care (Basel)] 2010 Mar; Vol. 5 (1), pp. 7-10. Date of Electronic Publication: 2010 Feb 16. |
| DOI: |
10.1159/000272241 |
| Abstrakt: |
BACKGROUND: Proteomic analysis has become an effective tool in breast cancer research. In this study, we applied the new gel-free tandem mass tag (TMT) reference method for the first time in breast cancer. MATERIALS AND METHODS: Proteomic analysis was used to compare 10 estrogen receptor (ER)-positive and 10 ER-negative samples. The results of the proteomic approach were validated by Western blot, immunohistochemistry and gene expression analysis. RESULTS: 17 proteins with significant differences in expression were identified. 13 proteins were overexpressed in ER-negative tumors and 4 were overexpressed in ER-positive samples. All these proteins were characterized by relatively high cellular abundance. CONCLUSIONS: Our results demonstrate that the gel-free TMT approach allows the quantification of differences in protein expression levels. Further improvement of the sensitivity by subfractionation of the tissue should allow also the identification of low-abundance proteins and might lead to the use of this method in breast cancer research. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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