Inflammation induced by phytomodulatory proteins from the latex of Calotropis procera (Asclepiadaceae) protects against Salmonella infection in a murine model of typhoid fever.

Autor: Oliveira RS; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, Cx., Postal 6033, Fortaleza, Ceará, CEP 60451-970, Brazil., Figueiredo IS, Freitas LB, Pinheiro RS, Brito GA, Alencar NM, Ramos MV, Ralph MT, Lima-Filho JV
Jazyk: angličtina
Zdroj: Inflammation research : official journal of the European Histamine Research Society ... [et al.] [Inflamm Res] 2012 Jul; Vol. 61 (7), pp. 689-98. Date of Electronic Publication: 2012 Apr 10.
DOI: 10.1007/s00011-012-0460-8
Abstrakt: Objective and Design: Laticifer proteins (LP) of Calotropis procera were fractionated by ion-exchange chromatography, and the influence of a sub-fraction (LP(PI)) on the inflammatory response of Swiss mice challenged by Salmonella enterica Ser. Typhimurium was investigated.
Methods: Mice (n = 10) received LP(PI) (30 or 60 mg/kg) in a single inoculum by the intraperitoneal route 24 h before infection. To investigate the relevance of the proteolytic activity, three additional groups were included: the first one received heat-treated LP (30 mg/kg-30 min at 100 °C), the second received LP (30 mg/kg) inactivated by iodoacetamide, and a control group received only phosphate-buffered saline (PBS).
Results: The survival rate reached 100 % in mice treated with LP(PI) and was also observed with the other treatment, whereas the PBS group died 1-3 days after infection. The neutrophil infiltration into the peritoneal cavity of pretreated mice was enhanced and accompanied by high bacterial clearance from the bloodstream. Tumor necrosis factor-alpha mRNA transcripts, but not interferon-gamma, were detected early in spleen cells of pretreated mice after infection; however, the nitric oxide contents in the bloodstream were decreased in comparison to the PBS group.
Conclusions: The inflammatory stimulus of C. procera proteins increased phagocytosis and balanced the nitric oxide release in the bloodstream, preventing septic shock induced by Salmonella infection.
Databáze: MEDLINE
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