Staining membrane-bound proteins with colloidal gold.

Autor: Stochaj WR, Berkelman T, Laird N
Jazyk: angličtina
Zdroj: CSH protocols [CSH Protoc] 2006 Oct 01; Vol. 2006 (5). Date of Electronic Publication: 2006 Oct 01.
DOI: 10.1101/pdb.prot4545
Abstrakt: INTRODUCTIONColloidal Gold is the most sensitive staining technique for proteins bound on membranes, detecting as little as 1-3 ng of protein. Protein spots are permanently stained a dark red after incubation with the Colloidal Gold solution. Colloidal Gold staining can detect proteins on both nitrocellulose and PVDF membranes, but it is not recommended for nylon membranes.
Databáze: MEDLINE